The Rapid Carbapenemase Detection Method (rCDM) for Rapid and Accurate Detection of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa
نویسندگان
چکیده
منابع مشابه
Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes
Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carbapenemase enzymes. Materials and methods: A total of 97 clinical specimens was collected from pa...
متن کاملRapid Detection of Carbapenemase-producing Enterobacteriaceae
To rapidly identify carbapenemase producers in Enterobacteriaceae, we developed the Carba NP test. The test uses isolated bacterial colonies and is based on in vitro hydrolysis of a carbapenem, imipenem. It was 100% sensitive and specific compared with molecular-based techniques. This rapid (<2 hours), inexpensive technique may be implemented in any laboratory.
متن کاملEvaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa.
The Carba NP test was evaluated against a panel of 244 carbapenemase- and non-carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa isolates. We confirmed the 100% specificity and positive predictive value of the test, but the sensitivity and negative predictive value were 72.5% and 69.2%, respectively, and increased to 80% and 77.3%, respectively, using a more concentrated bact...
متن کاملRapid detection of carbapenemase-producing Pseudomonas spp.
A novel biochemical technique, the Carba NP test, has been evaluated to detect carbapenemase production in Pseudomonas spp. This test was specific (100%), sensitive (94.4%), and rapid (<2 h). This cost-effective test, which could be implemented in any microbiology laboratory, offers a reliable technique for identification of carbapenemase-producing Pseudomonas spp.
متن کاملStrategy for rapid detection of carbapenemase-producing Enterobacteriaceae.
A prospective survey was conducted on 862 Enterobacteriaceae isolates with reduced susceptibility to carbapenems. The Carba NP test, UV spectrophotometry, and a DNA microarray were used to detect carbapenemase producers, and the results were compared to those from PCR and sequencing. The 172 carbapenemase producers were detected using the Carba NP test and UV spectrophotometry, whereas the DNA ...
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ژورنال
عنوان ژورنال: Frontiers in Cellular and Infection Microbiology
سال: 2019
ISSN: 2235-2988
DOI: 10.3389/fcimb.2019.00371